Genetically modified cell lines

With our custom-made genetically modified cell lines, using the innovative CRISPR/Cas9 technology, we enable a multitude of genetic modifications to support your research. Our services encompass gene knockouts for functional studies, gene knock-ins for overexpression or rescue experiments, and precise gene modifications to investigate specific genetic variations. By providing these versatile options, we empower you to delve deeper into gene function, disease mechanisms, and potential therapeutic avenues.


Additional benefits:


Tailored DNA elements: We can introduce complex new genes into selected cells through personalized gene synthesis, 3D protein design, and a comprehensive database of vector backbone elements. This tailored approach, allows us to meet highly specific research requirements, even for complex gene knock-ins that seem unachievable elsewhere. Whether it's a unique protein structure you're investigating, novel fusion protein or a novel gene of interest, our technology can bring it to life in your chosen cell line.


Next-Generation Sequencing validation both the modified cell and the original cell: We add an additional layer of confidence to your research by using Next-Generation Sequencing (NGS) to sequence both the modified cell and the original cell, thereby identifying all the differences between the two. This comprehensive analysis assures you that any observed phenotypic change is a direct result of the desired mutation, ruling out any off-target effects that might confound your results. This level of precision not only bolsters the validity of your research but is also a powerful tool for substantiating your findings during the publication process.


Full money back guarantee: We understand the intricacies and challenges of the CRISPR/Cas9 system, acknowledging that it is a complex and risk-prone process that can sometimes result in failure. However, our expertise and confidence in our skills allow us to provide a full money back guarantee. If we fail to produce the ordered genetically modified cell, we promise a full refund of your payment. This assurance not only reflects our commitment to successful outcomes but also serves to minimize your financial risk.


Ready to use full materials and methods and publication data: Providing a ready-to-use, comprehensive materials and methods and descriptions is a key component of our service. Drafting a materials and methods section can be time-consuming and complex, potentially requiring several hours to days of a researcher's time depending on the complexity of the procedures used. By supplying a detailed, publication-ready description, we free up significant time for our customers.

Information requested when ordering a product:

Gene Knockout
Gene Knock-In


Examples Of What You Can Do

The subcellular localization of proteins is crucial in numerous biological processes, including signal transduction, gene expression, and cell division. Understanding a protein’s subcellular location can shed light on its function, regulation, and interaction with other cellular components. However, studying protein localization can present challenges, particularly as many proteins express at low levels, rendering them difficult to detect. Additionally, subcellular localization might be influenced by overexpression, non-optimized fusion tags, or other experimental manipulations.


CRISPR technology provides a powerful solution to these obstacles by facilitating precise endogenous gene tagging with optimized peptide linkers containing fluorescent proteins or other reporters. This allows the study of protein localization in its native context, offering more accurate and physiologically relevant insights compared to using exogenous promoters or non-optimized fusion tags, which may distort protein expression levels or localization.


CRISPR-mediated tagging of endogenous genes can be achieved with remarkable efficiency and specificity, enabling the production of stable cell lines expressing the tagged protein at physiological levels. This opens up opportunities for studying protein localization under various conditions, such as during development, in response to cellular stress, or during disease states.


A primary challenge in drug discovery involves identifying targets that are both effective and safe. Traditional drug screening techniques often employ cell lines or animal models that don’t fully represent the complexity of human diseases, leading to high failure rates in clinical trials. However, utilizing CRISPR technology to create disease-relevant cell models can enhance the success rate of drug discovery by pinpointing targets more specific to the disease in question.


CRISPR technology equips researchers with the means to create cell models featuring specific genetic mutations or modifications, like the knockout or knock-in of particular genes. This allows the study of their role in disease progression and drug response. Furthermore, CRISPR enables the rapid generation of a significant number of cell models with specific genetic modifications, thereby reducing the time and costs associated with drug screening. This enhancement in efficiency can expedite drug discovery, facilitating the quicker delivery of new treatments to patients.

Traditional methods of genetic manipulation frequently suffer from off-target effects, whereby unintended genes are impacted, leading to uncertainty in results. In contrast, CRISPR technology enables highly specific genetic modifications, allowing researchers to target an exact gene or region of interest without impacting other genes in the genome.


By utilizing CRISPR to generate knock-out or knock-in cell lines, researchers can inspect the effect of a particular protein in a more physiologically relevant context. This approach can yield invaluable insights into the protein’s mechanism of action and its role in biological processes, helping to expedite the research process.


Additionally, CRISPR can be employed to study the interaction of the protein with other proteins or cellular components. By tagging the protein with a fluorescent marker, researchers can examine its subcellular localization and identify its binding partners. This offers valuable insights into its mechanism of action.

One of the prime benefits of employing CRISPR technology to modify bacterial genes is the capability to create or optimize metabolic pathways. By adding, deleting, or modifying specific genes, researchers can construct new metabolic pathways or enhance existing ones. This strategy can facilitate the production of novel compounds such as biofuels, bioplastics, and pharmaceuticals, with heightened efficiency and specificity.


This innovation leads to the development of more efficient and cost-effective industrial processes for the production of a vast array of compounds. Furthermore, CRISPR offers precise control over gene expression in bacteria. By utilizing CRISPR to introduce inducible promoters or other regulatory elements, researchers can regulate the expression of specific genes in response to environmental or other stimuli. This control grants more influence over metabolic pathways, enhancing the efficiency of production processes.

Frequently Asked Questions

Genetically modified cell lines are cells that have been altered using molecular biology techniques to introduce, remove, or modify specific genes. These cell lines allow researchers to study gene function, disease mechanisms, and potential therapeutic targets.

Our custom-made genetically modified cell lines, using the innovative CRISPR/Cas9 technology, enable a multitude of genetic modifications. We offer services such as gene knockouts, gene knock-ins, and precise gene modifications to support your specific research needs.

Gene knockout is a powerful technique used to study gene function by completely removing the gene from the cell line. This allows researchers to understand what happens in the absence of that specific gene, providing crucial insights into its role and function.

Gene knock-ins allow for the introduction of a gene into a specific location in the genome. This can be useful for overexpression studies, rescue experiments, or for the investigation of specific genetic variations.

CRISPR/Cas9 is a groundbreaking technology used to make precise, targeted changes to the genome of a cell. It is used to create genetically modified cell lines due to its efficiency, precision, and flexibility, enabling a wide range of genetic modifications.

Yes, we can create genetically modified cell lines tailored to your specific research needs. This includes specific gene knockouts, gene knock-ins, and precise gene modifications.

Genetically modified cell lines can significantly enhance your research outcomes. The ability to study the function of specific genes or genetic variations can provide powerful insights, strengthening the impact and credibility of your research publications.

Absolutely, genetically modified cell lines are widely used to study disease mechanisms. By altering specific genes, researchers can investigate their role in disease development and progression, aiding in the discovery of potential therapeutic targets.

We use Next-Generation Sequencing (NGS) to validate our genetic modifications, confirming the presence and accuracy of the intended modification and ensuring the absence of off-target effects.

Yes, genetically modified cell lines are an invaluable tool in therapeutic development. They can be used to identify and validate therapeutic targets, test potential therapeutics, and investigate the effects of these therapeutics on specific genetic pathways.

Reach out to us if you have any queries about our products. We assure you of a response within 24 hours.

Shopping cart0
There are no products in the cart!