Create a DNA vector construct of your choice with us. From RNA interference and GFP-marked proteins to complex fusion proteins, we offer comprehensive assistance. Our services within molecular cloning range from identifying the most effective organism-specific promoter and selection marker, providing advanced 2D and 3D modeling to fine-tune the expression product and whole gene synthesis.
5-8 week delivery.
Free international shipping.
Custom gene synthesis, so that we can make exactly the DNA you need.
Model organism specific codon optimization for robust expression.
Next-Generation Sequencing validation.
A fully annotated vector map containing a sequence confirmed by Next-Generation Sequencing.
5 vials per plasmid so you never lose your DNA.
Designed by experts to meet your specific needs.
Tailored vector backbone elements: Having a comprehensive database of vector backbone elements before designing a complicated DNA vector is critical. These elements - including a range of promoters, expression control sequences, transcription terminators, protein linkers, and protein tags - each play a crucial role in the gene expression process, affecting the efficiency, localization, and control of the output. Access to a vast selection of these elements allows us to tailor the vector design to the specific needs of the project, optimizing for factors like expression strength, stability, or regulatory control.
Gene expression validation: Gene expression process is influenced by many factors including the promoter, terminator, and vector backbone used, all of which can affect the efficiency of gene expression. Each of these elements may interact differently with different genes or host organisms, leading to variability in expression levels. Therefore, verifying the expression level post-cloning is crucial to ensure the vector is functioning as intended and that sufficient protein or RNA is being produced for downstream applications.
3D structure prediction: Designing fusion proteins that retain their full functionality and biological activity is a complex task, and this is where our cutting-edge 3D structure prediction service steps in. This process is essential to ensure the proteins you're studying maintain their structure and function, even when fused with additional tags or proteins.
Ready to use full materials and methods and publication data: Providing a ready-to-use, comprehensive materials and methods and descriptions is a key component of our service. Drafting a materials and methods section can be time-consuming and complex, potentially requiring several hours to days of a researcher's time depending on the complexity of the procedures used. By supplying a detailed, publication-ready description, we free up significant time for our customers.